Perfusion‐Decellularization of Porcine Lung and Trachea for Respiratory Bioengineering
Identifieur interne : 000117 ( Main/Exploration ); précédent : 000116; suivant : 000118Perfusion‐Decellularization of Porcine Lung and Trachea for Respiratory Bioengineering
Auteurs : Alexander Weymann [Allemagne, Royaume-Uni] ; Nikhil Prakash Patil [Royaume-Uni] ; Anton Sabashnikov [Royaume-Uni] ; Sevil Korkmaz [Allemagne] ; Shiliang Li [Allemagne] ; Pal Soos [Hongrie] ; Roland Ishtok [Hongrie] ; Nicole Chaimow [Allemagne] ; Ines P Tzold [Allemagne] ; Natalie Czerny [Allemagne] ; Bastian Schmack [Allemagne] ; Aron-Frederik Popov [Royaume-Uni] ; Andre Rüdiger Simon [Royaume-Uni] ; Matthias Karck [Allemagne] ; Gabor Szabo [Allemagne]Source :
- Artificial Organs [ 0160-564X ] ; 2015-12.
English descriptors
- Teeft :
- Acellular, Acellular scaffold, Adjacent trachea, Airway, Airway compliance, Alexander weymann, Artif, Artif organ, Artificial organ, Bioartifical platform, Bioartificial, Bioartificial lung, Bioartificial scaffold, Biomechanical, Biomechanical integrity, Cardiac surgery, Cell debris, Collagen formation, Component such, Decellularization, Decellularization protocol, Decellularization treatment, Decellularized, Decellularized lung, Decellularized trachea, Dynamic compliance, Elastin, Extracellular matrix, Fluorescence microscopy, Heart transplantation, Higher ventilatory volume, Human lung, Lesser airway pressure, Lung, Lung tissue, Matrix, Maximal tensile strength, Mechanical agitator, Native lung, Native trachea, Obvious difference, Organ regeneration, Passive tensile strength, Peak pressure, Perfusion, Perfusion decellularization, Porcine, Porcine lung, Protein band, Protocol, Relevant change, Remnant nuclear structure, Respiratory tissue engineering, Room temperature, Same intensity, Scale bar, Sloughed cell material, Static compliance, Stress resistance similar, Structral integrity, Substantial stiffening, Tidal volume, Tissue resistance, Trachea, Weymann.
Abstract
Decellularization of native organs may provide an acellular tissue platform for organ regeneration. However, decellularization involves a trade‐off between removal of immunogenic cellular elements and preservation of biomechanical integrity. We sought to develop a bioartificial scaffold for respiratory tissue engineering by decellularization of porcine lungs and trachea while preserving organ architecture and vasculature. Lung–trachea preparations from 25 German Landrace pigs were perfused in a modified Langendorff circuit and decellularized by an SDC (sodium deoxycholate)‐based perfusion protocol. Decellularization was evaluated by histology and fluorescence microscopy, and residual DNA quantified spectrophotometrically and compared with controls. Airway compliance was evaluated by endotracheal intubation and mechanical ventilation to simulate physiological breathing‐induced stretch. Structural integrity was evaluated by bronchoscopy and biomechanical stress/strain analysis by measuring passive tensile strength, all compared with controls. Decellularized lungs and trachea lacked intracellular components but retained specific collagen fibers and elastin. Quantitative DNA analysis demonstrated a significant reduction of DNA compared with controls (32.8 ± 12.4 μg DNA/mg tissue vs. 179.7 ± 35.8 μg DNA/mg tissue, P < 0.05). Lungs and trachea decellularized by our perfusion protocol demonstrated increased airway compliance but preserved biomechanical integrity as compared with native tissue. Whole porcine lungs–tracheae can be successfully decellularized to create an acellular scaffold that preserves extracellular matrix and retains structral integrity and three‐dimensional architecture to provide a bioartifical platform for respiratory tissue engineering.
Url:
DOI: 10.1111/aor.12481
Affiliations:
- Allemagne, Hongrie, Royaume-Uni
- Bade-Wurtemberg, District de Karlsruhe, Hongrie centrale
- Budapest, Heidelberg
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Le document en format XML
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uniqKey="Simon A" first="Andre Rüdiger" last="Simon">Andre Rüdiger Simon</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Cardiothoracic Transplantation & Mechanical Circulatory Support, Royal Brompton and Harefield NHS Foundation Trust, Middlesex</wicri:regionArea><wicri:noRegion>Middlesex</wicri:noRegion></affiliation></author><author><name sortKey="Karck, Matthias" sort="Karck, Matthias" uniqKey="Karck M" first="Matthias" last="Karck">Matthias Karck</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Cardiac Surgery, Heart and Marfan Center, University of Heidelberg, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Szabo, Gabor" sort="Szabo, Gabor" uniqKey="Szabo G" first="Gabor" last="Szabo">Gabor Szabo</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Cardiac Surgery, Heart and Marfan Center, University of Heidelberg, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Artificial Organs</title><title level="j" type="alt">ARTIFICIAL ORGANS</title><idno type="ISSN">0160-564X</idno><idno type="eISSN">1525-1594</idno><imprint><biblScope unit="vol">39</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="1024">1024</biblScope><biblScope unit="page" to="1032">1032</biblScope><biblScope unit="page-count">9</biblScope><date type="published" when="2015-12">2015-12</date></imprint><idno type="ISSN">0160-564X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0160-564X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acellular</term><term>Acellular scaffold</term><term>Adjacent trachea</term><term>Airway</term><term>Airway compliance</term><term>Alexander weymann</term><term>Artif</term><term>Artif organ</term><term>Artificial organ</term><term>Bioartifical platform</term><term>Bioartificial</term><term>Bioartificial lung</term><term>Bioartificial scaffold</term><term>Biomechanical</term><term>Biomechanical integrity</term><term>Cardiac surgery</term><term>Cell debris</term><term>Collagen formation</term><term>Component such</term><term>Decellularization</term><term>Decellularization protocol</term><term>Decellularization treatment</term><term>Decellularized</term><term>Decellularized lung</term><term>Decellularized trachea</term><term>Dynamic compliance</term><term>Elastin</term><term>Extracellular matrix</term><term>Fluorescence microscopy</term><term>Heart transplantation</term><term>Higher ventilatory volume</term><term>Human lung</term><term>Lesser airway pressure</term><term>Lung</term><term>Lung tissue</term><term>Matrix</term><term>Maximal tensile strength</term><term>Mechanical agitator</term><term>Native lung</term><term>Native trachea</term><term>Obvious difference</term><term>Organ regeneration</term><term>Passive tensile strength</term><term>Peak pressure</term><term>Perfusion</term><term>Perfusion decellularization</term><term>Porcine</term><term>Porcine lung</term><term>Protein band</term><term>Protocol</term><term>Relevant change</term><term>Remnant nuclear structure</term><term>Respiratory tissue engineering</term><term>Room temperature</term><term>Same intensity</term><term>Scale bar</term><term>Sloughed cell material</term><term>Static compliance</term><term>Stress resistance similar</term><term>Structral integrity</term><term>Substantial stiffening</term><term>Tidal volume</term><term>Tissue resistance</term><term>Trachea</term><term>Weymann</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">Decellularization of native organs may provide an acellular tissue platform for organ regeneration. However, decellularization involves a trade‐off between removal of immunogenic cellular elements and preservation of biomechanical integrity. We sought to develop a bioartificial scaffold for respiratory tissue engineering by decellularization of porcine lungs and trachea while preserving organ architecture and vasculature. Lung–trachea preparations from 25 German Landrace pigs were perfused in a modified Langendorff circuit and decellularized by an SDC (sodium deoxycholate)‐based perfusion protocol. Decellularization was evaluated by histology and fluorescence microscopy, and residual DNA quantified spectrophotometrically and compared with controls. Airway compliance was evaluated by endotracheal intubation and mechanical ventilation to simulate physiological breathing‐induced stretch. Structural integrity was evaluated by bronchoscopy and biomechanical stress/strain analysis by measuring passive tensile strength, all compared with controls. Decellularized lungs and trachea lacked intracellular components but retained specific collagen fibers and elastin. Quantitative DNA analysis demonstrated a significant reduction of DNA compared with controls (32.8 ± 12.4 μg DNA/mg tissue vs. 179.7 ± 35.8 μg DNA/mg tissue, P < 0.05). Lungs and trachea decellularized by our perfusion protocol demonstrated increased airway compliance but preserved biomechanical integrity as compared with native tissue. Whole porcine lungs–tracheae can be successfully decellularized to create an acellular scaffold that preserves extracellular matrix and retains structral integrity and three‐dimensional architecture to provide a bioartifical platform for respiratory tissue engineering.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Hongrie</li><li>Royaume-Uni</li></country><region><li>Bade-Wurtemberg</li><li>District de Karlsruhe</li><li>Hongrie centrale</li></region><settlement><li>Budapest</li><li>Heidelberg</li></settlement></list><tree><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Weymann, Alexander" sort="Weymann, Alexander" uniqKey="Weymann A" first="Alexander" last="Weymann">Alexander Weymann</name></region><name sortKey="Chaimow, Nicole" sort="Chaimow, Nicole" uniqKey="Chaimow N" first="Nicole" last="Chaimow">Nicole Chaimow</name><name sortKey="Czerny, Natalie" sort="Czerny, Natalie" uniqKey="Czerny N" first="Natalie" last="Czerny">Natalie Czerny</name><name sortKey="Karck, Matthias" sort="Karck, Matthias" uniqKey="Karck M" first="Matthias" last="Karck">Matthias Karck</name><name sortKey="Korkmaz, Sevil" sort="Korkmaz, Sevil" uniqKey="Korkmaz S" first="Sevil" last="Korkmaz">Sevil Korkmaz</name><name sortKey="Li, Shiliang" sort="Li, Shiliang" uniqKey="Li S" first="Shiliang" last="Li">Shiliang Li</name><name sortKey="P Tzold, Ines" sort="P Tzold, Ines" uniqKey="P Tzold I" first="Ines" last="P Tzold">Ines P Tzold</name><name sortKey="Schmack, Bastian" sort="Schmack, Bastian" uniqKey="Schmack B" first="Bastian" last="Schmack">Bastian Schmack</name><name sortKey="Szabo, Gabor" sort="Szabo, Gabor" uniqKey="Szabo G" first="Gabor" last="Szabo">Gabor Szabo</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Weymann, Alexander" sort="Weymann, Alexander" uniqKey="Weymann A" first="Alexander" last="Weymann">Alexander Weymann</name></noRegion><name sortKey="Patil, Nikhil Prakash" sort="Patil, Nikhil Prakash" uniqKey="Patil N" first="Nikhil Prakash" last="Patil">Nikhil Prakash Patil</name><name sortKey="Popov, Aron Rederik" sort="Popov, Aron Rederik" uniqKey="Popov A" first="Aron-Frederik" last="Popov">Aron-Frederik Popov</name><name sortKey="Sabashnikov, Anton" sort="Sabashnikov, Anton" uniqKey="Sabashnikov A" first="Anton" last="Sabashnikov">Anton Sabashnikov</name><name sortKey="Simon, Andre Rudiger" sort="Simon, Andre Rudiger" uniqKey="Simon A" first="Andre Rüdiger" last="Simon">Andre Rüdiger Simon</name></country><country name="Hongrie"><region name="Hongrie centrale"><name sortKey="Soos, Pal" sort="Soos, Pal" uniqKey="Soos P" first="Pal" last="Soos">Pal Soos</name></region><name sortKey="Ishtok, Roland" sort="Ishtok, Roland" uniqKey="Ishtok R" first="Roland" last="Ishtok">Roland Ishtok</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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